FastQCFastQC Report
Fri 29 Apr 2016
ERR1042714.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042714.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9050595
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT199060.2199413408731691No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT182630.20178783825814767No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT128360.14182492974218822No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG20100.015.18656635
TAACGGC21150.014.08274336
CTCTAAT27800.013.9082741
CTAATAC33800.013.8476323
TAATACT34200.013.79385954
GTATTAG32500.013.7184611
GGTAAAC25950.013.473989535
ATTAGAG24400.013.4200813
TTTAACG23350.013.31049334
TATTAGA28950.013.2279792
TCTAATA30050.013.1747092
AATACTG33400.013.0164675
ATCTCGT12100.012.84297537
GGGGTTA16150.012.8297216
AACGGCC24000.012.7187537
TAGGACC20700.012.5120764
ATGCTAG27950.012.377460515
GTTTAGG24100.012.3589221
TAGGGGT16650.012.3333334
ATACTGG34750.012.1913676
Produced by FastQC (version 0.11.5)