FastQCFastQC Report
Fri 29 Apr 2016
ERR1042713.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042713.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3997629
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG115490.28889624324818536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT103790.25962889502752756No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG97680.24434483540118407No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG88830.22220671302914802No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT88280.22083089751450172No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG84150.2104997737408849No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG69880.174803614842698No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT67250.16822471519993476No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60630.15166489936910105No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG58860.14723727489469382No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA54980.1375315218095526No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48660.1217221508048896No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA47800.11957087563653356No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT46020.11511823633458733No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA41810.10458699394065832No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC5200.014.2307683
GGACCGT2051.4279067E-913.5365866
GTATTAG14500.013.5241381
TACTCCG15550.012.7299045
TTAGACT3200.012.7187514
TTACTTA5000.012.5829
TTAACGG9900.012.33333435
ATTAGAG10850.012.105993
TATTAGA12700.012.090552
TCGTATG3250.011.95384737
TACCGAC1552.7236398E-511.93548333
GGGTAAG4500.011.9222221
CGTATTA2808.367351E-1111.89285715
TACTTAT5300.011.86792530
GTATCGT1100.003747830911.7727276
CTAATAC14150.011.7667853
TCACGTA12900.011.6162825
TAACCCG1603.768115E-511.5625015
AGGTCGC10900.011.54128535
TAGGTCG2103.848345E-711.452385
Produced by FastQC (version 0.11.5)