FastQCFastQC Report
Fri 29 Apr 2016
ERR1042712.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042712.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4094051
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT221080.540003043440348No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT194210.47437122791093705No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94360.23048076342966906No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86560.2114287291487087No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG43380.10595862142411025No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG43110.10529912792976931No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC41200.10063382209943159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG4950.016.4444455
TACCGAT700.00259401515.8571427
GTAAGAT8150.014.7546013
TGCTCGG14500.014.41724210
GGGTAAG8350.014.1796411
AGGTCGC12800.013.585937535
CCGAGTT6550.013.55725213
TGTATAC4200.013.2142853
TACACAG10050.013.0696535
GCTCCGA13350.012.749063529
GGTCGCC14050.012.6405736
GTATACT4100.012.6341474
TTACTTA6800.012.51470629
CTATCTA7000.012.42142820
GCTCGGA15000.012.33333411
TCACGTA11850.012.33333325
GTCGCCC14200.012.24647937
ACTGCTC19300.012.1735758
TGTACAC5950.012.126053
CTGCTCG17550.012.1225079
Produced by FastQC (version 0.11.5)