FastQCFastQC Report
Fri 29 Apr 2016
ERR1042709.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042709.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4688255
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT457420.9756721850667253No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG265390.5660741576556736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT245110.5228171249217459No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205960.4393105750433797No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG186490.3977812640310734No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG185690.3960748722072498No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT176920.37736855183858387No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG141950.3027778992396958No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT136850.29189965136282053No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT135480.28897745536452263No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG115130.2455711133460104No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT109680.2339463190462123No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG107660.22963767969105775No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA96850.20658006017164168No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA84650.18055758485833215No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA76710.16362164600688317No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68320.1457258617545334No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC67550.1440834596241032No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC65400.13949753159757736No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG61960.13216004675513596No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT61540.1312641910476286No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG58190.12411867528536738No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT56920.12140977826504745No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT54370.11597065432660979No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG54360.115949324428812No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA51000.10878247876875298No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC49030.10458048890258742No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACGCT2700.015.07407330
ATACCCG1406.005721E-714.5357155
TACGCTA3000.012.94999931
GTATTAG8050.012.6397511
TCTAGAC4100.012.6341473
ATAGGAC3600.012.3333343
ACGCTAC3300.012.33333427
CCGTTAA1951.4704528E-712.33333416
CGTACCT3650.012.16438433
GACGCTA3400.011.97058926
GTAGCAC4050.011.8765433
CGAGACG4050.011.87654323
CGCTACG3600.011.81944528
GCTACGC3450.011.79710129
GCTAGAC2552.9376679E-911.6078433
CGAACTA2257.562085E-811.51111124
CTAGACA5050.011.3564354
CGCGAAA1801.0047637E-511.30555615
GTACTAG2655.3678377E-911.1698111
TCTAGCC4150.011.1445783
Produced by FastQC (version 0.11.5)