FastQCFastQC Report
Fri 29 Apr 2016
ERR1042708.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042708.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6525195
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG850881.3039916814746533No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG618230.9474506125870568No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG559920.858089298480735No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT515620.7901986070914355No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT510760.7827505538148668No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG416610.6384636780969765No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG368640.5649486337189923No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT339750.520674094797167No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG312740.4792806958259485No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA245470.3761879913167346No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG232630.35651041846259No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA231790.3552231006123189No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA221390.33928487960896186No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC215990.3310092648572188No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG191260.2931100143367363No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA189670.29067330554872306No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC186320.2855393593601417No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG173610.2660610142685391No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT159910.2450654731391169No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT158950.24359425273880703No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT146480.22448371274728188No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCT144860.22200102832175897No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGC134530.20617008380592458No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC120790.18511324182648947No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG118980.18233937836340525No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC108110.16568087237239654No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA103550.1586925754709246No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG102510.15709875337058893No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG100330.15375785704488523No Hit
ACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC99890.15308354769474322No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCC94900.14543626665563253No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGT92060.1410839063047158No Hit
GTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTT91880.1408080524796577No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC90540.13875447400422516No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA89710.13748248136645724No Hit
TCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCA82710.12675483261419773No Hit
CTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGC81540.12496178275132007No Hit
GCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCATCAATA80700.12367446490104894No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT79470.12178946376315192No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCG79380.12165153685062285No Hit
GTAGTGCGCTATGCCGATCGGGTGTCCGCACTAAGTTCGGCAT75290.11538352493680266No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74230.1137590524114605No Hit
GCCTAAGGAGGGGTGAACCGGCCCAGGTCGGAAACGGAGCAGG72700.11141429489846663No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTAT71800.11003502577317611No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA70450.10796612208524037No Hit
AAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC68630.1051769334096529No Hit
TGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTC66640.10212721612151054No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC22200.025.753
TTATACA24100.023.105812
CTTATAC25050.022.4510971
TACACAT31050.018.8276965
ATACACA32500.018.7276924
ACACATC38600.015.4805696
CGTATAC2201.8189894E-1215.1363643
ACATCTC41050.014.8270418
ATCTCCG41450.014.01447610
GTACTAG3050.013.950821
CCGTCTT13450.013.75464637
ATCTCGT41650.013.50300137
AATCTCG42550.013.47826136
CACATCT44400.013.4583337
ACGAGAC43800.013.30479522
CGAGACA43800.013.17808223
CACGAGA43550.013.12629121
GCCGTCT15100.012.98675436
TCTCCGA45150.012.82502711
ACGTTTA1307.007583E-512.80769326
Produced by FastQC (version 0.11.5)