FastQCFastQC Report
Fri 29 Apr 2016
ERR1042707.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042707.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4039843
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG67210.1663678514239291No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG58400.14456007325037137No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG54260.13431215024940327No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT42390.10492982029252126No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT40740.10084550315445427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG4050.015.5308645
TTAACGG8050.014.24844735
TAACGGC8650.013.04624436
CGAACTA8400.012.99404724
GGGTAAG4450.012.887641
TATTAGA10700.012.7943932
ATTAGAG10200.012.6960783
GTATTAG12550.012.52988151
CGTATTA1359.972041E-512.33333415
CCGATAA1205.12639E-412.3333349
TCTAGCA7800.012.3333343
TTACACT3200.012.1406254
TTTAACG9650.012.07772134
CGATAGA4000.012.02500137
TCACGTA7850.011.7834425
GTTTAAC9750.011.76410233
GTAGCAC4750.011.6842113
GTTTACC5450.011.54128515
GTATACT3053.092282E-1111.52459054
GCGAACT9800.011.515305523
Produced by FastQC (version 0.11.5)