FastQCFastQC Report
Fri 29 Apr 2016
ERR1042706.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042706.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1558532
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG46370.29752356704899224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT44610.28623088906740446No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG44500.2855250966935552No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40480.2597315935765194No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG39250.2518395515780234No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT35160.22559690785944722No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG29890.19178303685776102No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA26850.17227750216229118No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA26440.16964682149612584No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA22800.1462915102160238No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG21750.13955440119291743No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20350.13057158916210895No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT20010.12839004909748403No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC18330.11761067466051388No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG17450.11196433566971996No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC17350.11132270623894792No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG16790.10772958142662453No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC16190.10387980484199233No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT15980.10253238303737106No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT15880.10189075360659904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCCGA5650.019.64601729
TGCTCGG6300.017.32539710
CCGAGGT5850.017.07692332
GTCGCCC5900.016.93220337
TCTATCG1901.8189894E-1216.55263129
ACAGCGT1708.54925E-1116.323538
CGCCTAT1851.8189894E-1116.034
GTAAGAT2900.015.9482763
AGGTCGC6050.015.90082735
TAACCCG700.002593048415.8571435
CTATCGC2100.015.85714230
TACACAA2700.015.7592585
ACTGCTC7900.015.6898738
GCTCGGA6750.015.62222211
TGTACTG7600.015.5789485
TGCTCCG7100.015.3732428
CTGCTCG7600.015.3355269
GCATGTA7050.015.2198572
GGGTAAG2800.015.1964291
GTAACAC1853.0559022E-1015.03
Produced by FastQC (version 0.11.5)