FastQCFastQC Report
Fri 29 Apr 2016
ERR1042703.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042703.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3595749
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT558591.5534732819226258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT321330.893638571546568No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT226030.6286033869438606No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT191360.5321839761340404No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG114060.31720790299879104No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT77750.21622755092193588No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG74110.20610448615851662No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG70830.19698260362444653No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA68360.19011338110641204No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64570.17957315708076396No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT58070.16149625571751533No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG55860.15535010925401077No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT53100.14767437882900059No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA51450.14308562694448362No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC48740.13554894960688302No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG48540.1349927372572446No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG48100.13376907008804007No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT46380.12898564388114964No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46310.12879096955877622No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA42270.11755548009608012No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG41840.11635962354435753No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC41250.11471879711292417No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG41230.11466317587796034No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG40910.11377323611853887No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC40710.11321702376890044No Hit
CCCCTATACCTTCCGCATAATGAATTAACTAGAAATAACTTTG37510.10431762617468571No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT37280.10367798197260154No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA9100.020.1263736
TAGGGGT9400.018.3031924
GGGTTAG9850.017.842647
ATAGGGG9750.017.456413
GCCGAGT4600.017.29347612
TAATCCG752.068809E-417.2666665
CTAGACG650.001580818217.0769234
GTATTAG16400.016.5823151
TAACGGC12550.016.5099636
ATTAGAG14350.016.3728223
TTAACGG12700.016.3149635
TTTAACG13800.015.95289934
GTGACAC13600.015.91544124
ACCGCCT13850.015.76173312
AGGGGTT11000.015.6409095
CTTGCTA10000.015.5416
GACACAT14350.015.34146326
GGCACCG14350.015.3414639
TATTAGA16200.015.18827152
GTTTAAC14500.015.18275833
Produced by FastQC (version 0.11.5)