FastQCFastQC Report
Fri 29 Apr 2016
ERR1042702.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042702.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3728640
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT156090.4186244850669413No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT131490.3526486869207003No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT81460.21847107792653622No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58460.15678638860281496No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG37430.10038512701682115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGTTA7100.018.7605656
GGGTTAG8050.016.316777
TAGGGGT7900.015.924054
GTATTAG13050.014.8850581
ATAGGGG8700.014.2471273
TTAACGG7650.013.78431335
GGGTAAG5000.013.691
GGTTAGT9200.013.6739138
TACCCCG4850.012.9690725
AGGGGTT9850.012.9593915
TAACGGC8350.012.85029936
CCGAGTT4200.012.77380913
GCTATAT8600.012.69186119
TAATACT12150.012.4855974
GTTTAGG8950.012.4022351
TAGTCCT9100.012.40109911
GGTTATA9250.012.433
GTATAGG12600.012.3333341
GTACGAC1050.002645487712.3333333
CTATATT9350.012.26737920
Produced by FastQC (version 0.11.5)