FastQCFastQC Report
Fri 29 Apr 2016
ERR1042701.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042701.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3794520
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT235010.6193405226484509No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT185160.4879668574681383No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT117390.3093671926884033No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93800.24719859165322622No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG42630.11234622560956327No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGGGT6900.014.478264
TTAACGG9300.014.12365735
GGGTTAG6300.014.0952387
GGGGTTA6450.014.0542646
TAACGGC9600.013.87499936
GTATTAG13150.013.7870721
ATAGGGG6850.013.5036513
TAACGCC1102.4586965E-413.4545454
ATTAGAG12000.013.41253
TTTAACG10800.013.18981434
TAATACT12350.013.1821864
TATTAGA13150.013.0836512
CCCCTAT9900.013.0808091
CCGTTCG850.00940994713.0588249
TCTAATA9950.013.0150762
TAACTAG8400.012.77380926
CTACACT6900.012.6014494
AACTAGA8700.012.54597727
AACGGCC11100.012.33333337
CTATACC10850.012.2764984
Produced by FastQC (version 0.11.5)