FastQCFastQC Report
Fri 29 Apr 2016
ERR1042700.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042700.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384598
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT134520.39744749598032025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT115530.3413403896120012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76080.2247829727489055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54450.16087582631674424No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG51730.15283942140248266No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT41010.12116653144627516No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG36440.10766418936606356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG7500.017.75999835
TAGGGGT6050.017.4297524
TAACGGC7900.016.8607636
GGGGTTA6350.015.4409456
ATAGGGG6850.015.1240873
GGGTTAG6650.015.0225577
TTTAACG9800.014.53571434
TATTAGA12350.014.3805672
GTGACAC10200.014.32843124
ATTAGAG12150.014.31275753
AACGGCC9750.014.2307737
GTTTAAC10400.014.23076933
GTATTAG14700.013.9693871
AACTAGA8650.013.687860527
CCCCTAT9650.013.4196881
TATACCT10050.013.2537325
CGATAGA4750.013.242105537
GACCGAT1551.8904102E-613.12903219
ACCGCCT11750.013.06808612
ATACCTT10250.012.9951226
Produced by FastQC (version 0.11.5)