FastQCFastQC Report
Fri 29 Apr 2016
ERR1042699.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042699.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3043710
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT211810.695894155487875No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT129040.42395629018533304No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG118210.38837471375393845No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT103310.33942129834971135No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG82050.26957233113535783No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78430.25767895101701543No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG77620.2550177250789333No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG74570.24499705950961162No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG73410.24118592112914833No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT73050.24000315404555628No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC72170.23711194561899787No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG62300.2046844147438488No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT62040.20383019407236563No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT61760.2029102641184607No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG59850.1966350276471806No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC55760.1831974793919263No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT55690.18296749690345007No Hit
CCCCTATACCTTCCGCATAATGAATTAACTAGAAATAACTTTG54110.17777646359212934No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG53560.17596945832553038No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC53310.17514809229525807No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA42620.14002648084081598No Hit
CTACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAG42290.13894227768085657No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC41910.13769380131484274No Hit
GTCTTAGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGT41860.1375295281087883No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG40510.13309415154531806No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC38950.1279688275164191No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGA38880.12773884502794286No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA38180.12543902014318054No Hit
CCTCTAGCATCACCAGTATTAGAGGCACCGCCTGCCCAGTGAC38060.12504476444864984No Hit
GCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAAC37500.12320490454083995No Hit
ATCTATCACCCTATAGAAGAACTAATGTTAGTATAAGTAACAT37310.12258066635783303No Hit
GGCTTATGCGGAGGAGAATGTTTTCATGTTACTTATACTAACA36880.12116791678576475No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCAT35790.11758676089377766No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA35140.11545120921506975No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT34310.11272427399456585No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG34220.11242858222366783No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA33700.1107201408807015No Hit
ATCTTACCCCGCCTGTTTACCAAAAACATCACCTCTAGCATCA32520.10684329321781641No Hit
ATCCTATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTC32390.10641618288207484No Hit
ATTCTAGAGTCCATATCAACAATAGGGTTTACGACCTCGATGT32190.10575909005785702No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGGAAGATTTG32130.10556196221059168No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT32050.10529912508090455No Hit
AGGGATAACAGCGCAATCCTATTCTAGAGTCCATATCAACAAT31840.10460917761547586No Hit
GTCCTAAACTACCAAACCTGCATTAAAAATTTCGGTTGGGGCG31660.10401779407367981No Hit
ACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACA31550.10365639302036002No Hit
GTTATCCCTAGGGTAACTTGTTCCGTTGGTCAAGTTATTGGAT31000.10184938775376104No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCA30910.10155369598286303No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA30890.10148798670044122No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG12700.019.5196867
GGGGTTA12950.019.4285726
TAGGGGT12100.018.8057864
GGGTAAG6450.017.7829461
ATAGGGG13950.017.1075273
GCCGAGT6450.016.9224812
AGGGGTT13650.016.670335
TTAACGG15650.016.6677335
CTTGCTA13450.016.50557516
TAGGTCG1708.54925E-1116.32352821
CGACTAT1251.660228E-716.2836
CTCTAAT18750.016.281
CCGAGTT7200.016.187513
CGAGTTC7350.016.10884514
TAACGGC16250.016.05230736
GTAGCAC1504.6802597E-916.0333333
GTGACAC16750.016.01492524
CTAGTAC2700.015.7592583
GTAAGAT8250.015.696973
TAACTAG13800.015.68478326
Produced by FastQC (version 0.11.5)