FastQCFastQC Report
Fri 29 Apr 2016
ERR1042698.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042698.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3489589
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT150040.42996467492303536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT126130.3614465772330208No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76920.22042710473926871No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61710.17684030984737745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTAAAC8150.014.52760735
GTATTAG9700.014.3041241
CTCTAAT9700.014.1134021
TTAACGG5750.013.83478335
TAGGGGT6500.013.6615384
TAATACT11250.013.6488894
TAACGGC5900.013.4830536
CTAATAC11950.013.1589963
GGGGTTA5950.013.0588236
TCTAATA10150.012.94088652
TACTGGT10850.012.7880187
TCGAACG1050.002645428312.3333343
TAATCGG1050.002645428312.33333416
AACCGTA1501.9461037E-512.3333337
ATGCTAG9150.012.33333315
AATACTG12950.012.2857145
TATACTG5000.012.215
TACCCCG4700.012.2021285
ATTAGAG8200.012.1829263
GTTATAC2901.2732926E-1112.1206893
Produced by FastQC (version 0.11.5)