FastQCFastQC Report
Fri 29 Apr 2016
ERR1042697.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042697.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3335627
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT492291.4758544645429479No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG401771.2044811964886961No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT280550.8410712588667738No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG277500.8319275506523961No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT260140.77988336225843No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG258960.7763457964574576No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG213500.6400595750064381No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194380.5827390172822081No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG181370.5437358553579282No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT178560.5353116520522229No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA162710.4877943487086536No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG158650.47562272400361305No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA134500.40322254256845863No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT133500.4002246054489906No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT133110.399055409972398No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA118230.35444610563471274No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT96270.28861140649119343No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC94600.2836048515016817No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG94400.2830052640777881No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT94000.28180608923000083No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC93230.2794976776480104No Hit
TATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTAC85120.25518440760912414No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG79850.2393852789895273No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA67360.20194104436737081No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG63100.18916983223843672No Hit
ACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAG60180.1804158558495899No Hit
CCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTA59550.178527155464325No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58890.17654851696547605No Hit
GTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGA56770.1701928902722037No Hit
GCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGC55880.16752472623587708No Hit
GCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACG54340.16290790307189623No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT52220.15655227637862387No Hit
GTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCT51670.1549034109629164No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG51560.15457363787977493No Hit
ACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTGATACC48790.1462693520588483No Hit
ACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGT48310.14483034224150362No Hit
ATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCC47960.14378106424968978No Hit
CAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCA47560.14258188940190256No Hit
GCTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCG46760.14018353970632808No Hit
GTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGTA44460.13328828433155146No Hit
CTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTC44310.13283859376363125No Hit
AAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAAC43970.1318192951430121No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTAT43190.129480904189827No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA42910.1286414817963759No Hit
GATACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCC41320.12387476177642164No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC40690.12198606139115675No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG40660.12189612327757271No Hit
GTTGATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTT40160.12039715471783864No Hit
AGCATGTACTGCTCGGAGGTTGGGTTCTGCTCCGAGGTCGCCC39250.1176690319391227No Hit
GAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGTATCAA38590.11569039344027374No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTT37430.11221278638169077No Hit
TGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTCAATTG36980.11086371467793012No Hit
GGATTACTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCG34370.10303909879611839No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34350.10297914005372903No Hit
ATACCACTGCTTCCGTACTCTGCGTTGATACCACTGCTTCCGT34180.10246949074341945No Hit
ATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTAC33810.10136025400921625No Hit
TACCACTGCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCG33800.10133027463802159No Hit
AACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGG33550.10058079035815456No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTAAT400.001931953523.1251
GTAAGAT5500.019.8454553
GGGTAAG5550.019.3333341
GCCGAGT5100.018.86274512
ATAGTCG1156.4157575E-817.69565230
CCCACGA2800.016.51785719
ATGTACG1251.6603735E-716.2811
CTCTAAT12250.016.1591841
CTAGTAC1851.8189894E-1116.03
TATACTG1851.8189894E-1116.05
ATCTCGT2550.015.96078437
CCGAGTT5950.015.85714313
GGTAAGA6550.015.8167932
TTACTTA6050.015.59504129
TCTATAC2500.015.5400013
ATTAGAG11800.015.5211873
AATACTG14000.015.3285715
TTTGCCG6300.015.2698429
ATCTCCG3050.015.16393510
TAATACT14950.014.9732444
Produced by FastQC (version 0.11.5)