FastQCFastQC Report
Fri 29 Apr 2016
ERR1042696.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042696.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5142852
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT460330.8950870061981173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT317750.6178478400700623No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG255140.49610605166160715No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT188500.36652814430592207No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT187070.3637475859698082No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180520.3510114621225732No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG168370.3273864384975496No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG151700.2949725171947394No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG137270.2669141558030447No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT119990.2333141222030111No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT117170.22783078338633897No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG105580.20529464973909417No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG98220.19098352431685767No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA87540.17021683688350353No Hit
AAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAA69350.1348473570695793No Hit
ACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGA61840.12024456468900913No Hit
GCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATACCACTG59740.11616122727233838No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT57240.1113001113001113No Hit
GTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGT56510.10988066543622099No Hit
ACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTTGATAC54890.10673066228621783No Hit
GCTTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTG53910.10482510482510482No Hit
GTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAG52480.10204454648899093No Hit
CAGTGGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGC51730.10058621169732282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACGGC9150.015.16393536
GGACCGT2850.014.9298246
TTAACGG9400.014.76063935
CGTATCG1203.3047432E-513.87500110
CGCGCGA800.006302547613.87514
GTATTAG16000.013.6437491
TTTAACG10700.013.14018634
TAATACT14200.013.0281694
AACGGCC11250.012.99111237
CGTATTA1307.007025E-512.80769315
TAGGGGT7700.012.7337664
CTAGTAC3350.012.7014923
TCTAATA13850.012.4223822
GGGTAAG6500.012.2384621
TACCCCG5800.012.1206895
CTCTAAT13050.011.7662841
TTAGGAC9000.011.7166673
CCTCTAG12100.011.4669431
GTGCTAG5650.011.4601771
GGGGTTA9700.011.4432996
Produced by FastQC (version 0.11.5)