FastQCFastQC Report
Fri 29 Apr 2016
ERR1042695.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042695.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4089330
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT163770.4004812524301047No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT130690.31958780533730463No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG112340.27471492885142557No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT80410.19663367837763154No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT78730.19252542592551836No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG78450.19184071718349952No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG68330.16709338693624629No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64660.15811881163907046No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG56820.13894696686254226No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT56400.137919903749514No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT51510.1259619546478274No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG45990.11246341087659836No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG7750.014.56129135
GTATTAG13050.014.459771
TAACGGC7850.014.14012736
GGGGTTA7100.014.0704226
TATACTG5300.013.2641525
GTACTAG2001.4657417E-812.9500011
GGGTTAG7000.012.9500017
ATAGGGG6800.012.7867653
CTAGGTA2204.094545E-912.6136364
AACGGCC8900.012.4719137
TAATACT11100.012.3333344
TATTAGA12300.012.1829272
TAGGGGT6450.012.0465124
TAACGCC1705.3172953E-611.9705894
TTTAACG9900.011.77272634
ATTAGAG11200.011.7276783
CTAGTAC2552.935849E-911.6078443
TTAGGAC6450.011.4728683
TAATACC4050.011.4197534
AATACTG13100.011.1564885
Produced by FastQC (version 0.11.5)