FastQCFastQC Report
Fri 29 Apr 2016
ERR1042694.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042694.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4260505
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT425750.9992946845503057No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT299630.7032734382426497No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT198200.4652030686503126No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191010.4483271349288406No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG147440.3460622625721599No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG95190.22342421849053107No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT94050.2207484793469319No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG81830.19206643343922847No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT69520.16317314496755667No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG65610.15399582913293144No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT57990.13611062538361063No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57900.13589938281964226No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG55970.13136940339232087No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG52870.12409327063341083No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA50890.11944593422610698No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA49740.11674672368651134No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT49530.11625382437058518No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46440.10900116300767161No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAACGG6150.015.64227635
CTAATAC14300.014.2307693
TAACGGC6950.013.5755436
TAATACT12300.013.5365854
TAGGGGT6800.013.3308824
GTATTAG11550.013.2943731
TCTAATA11700.013.2820512
CTACACT8050.012.8695654
GGACCGT2305.7661964E-1012.8695656
TACTGGT12450.012.7791177
ATAGGGG7100.012.5070423
TCTACAC5050.012.4554463
TTTAACG8050.012.40993834
ATTAGAG9550.012.3979063
CCCCTAT7650.012.3333331
ATACTGG13650.012.3333336
GGGGTTA7400.012.256
TATACTG5650.012.1150455
CTCTAAT11150.012.1121071
GTACTAG2451.5643309E-912.0816331
Produced by FastQC (version 0.11.5)