FastQCFastQC Report
Fri 29 Apr 2016
ERR1042692.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042692.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4025279
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT224990.5589426223623257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT161220.40051882117984866No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT99810.24795796763404476No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG96350.23936229016671887No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83130.20651984620196512No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG68110.1692056625143251No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT65410.16249805292999567No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG60120.14935610674440206No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT48190.11971840958105016No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG47900.11899796262569626No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT41350.10272579863408225No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGATA650.001580863317.07692114
TTAACGG5200.016.00961535
TAACGGC5750.014.47826136
TAGGGGT5600.014.2053574
CCCCTAT7650.014.0261441
CCTCTAG7650.012.8169941
GTATAAA7050.012.5957451
GGGGTTA6400.012.42968756
CCTATAC10500.012.1571443
AATACTG12400.011.9354845
TTTAACG7750.011.93548334
GACCGTA2951.6370905E-1111.91525526
GTCTATA2808.367351E-1111.8928571
GTAGCAC3002.3646862E-1111.7166663
GTATTAG11400.011.684211
TATAGTG4600.011.6630445
TACACTG7950.011.635225
AACGGCC7350.011.57823137
CTCTAAT10400.011.5624991
CGTACTA3401.8189894E-1211.4264729
Produced by FastQC (version 0.11.5)