FastQCFastQC Report
Fri 29 Apr 2016
ERR1042690.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042690.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8409753
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT366280.43554192376399165No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT358260.4260053773279667No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT244260.2904484828508043No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG135660.16131270442782325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT130820.15555748189037183No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT108220.12868392210805715No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG87000.10345131420625553No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT12850.017.13229637
CCCACGA14900.015.89261719
CGCGTAA1557.228664E-915.516129510
ACGAGAC15300.015.35620922
CACGAGA15800.014.98734221
GGGGTTA14450.014.2110736
ATCTCCG16950.014.07964610
CGAGCCC17250.013.83478315
TCTCCGA17550.013.80911711
TAGGGGT14800.013.254
GTATTAG28350.012.985891
TATACAC22000.012.9500013
GCCCACG19200.012.71874918
GGACCGT4300.012.4767446
TACACAT24350.012.3080085
CGAACTA17150.012.08163324
GATCTCG18500.012.036
ATAGGGG16500.011.996973
ACACATC24100.011.9751036
TTAACGG15550.011.77813535
Produced by FastQC (version 0.11.5)