FastQCFastQC Report
Fri 29 Apr 2016
ERR1042688.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042688.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3414048
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT118400.3468023882499602No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT109550.3208800813579657No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT50340.14744959649073475No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG48180.14112279616455306No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT44500.13034380301624346No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG40580.11886183205391371No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37880.11095333164618658No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG35960.10532950913402506No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC8700.016.1609213
TATCGTA855.3679966E-415.23529427
CCCACGA8400.015.19642919
TATACTG4650.014.322585
ACGAGAC8400.014.31547722
CGAACTA8900.014.13483224
ATCTCGT8300.014.04216837
TACACAT11100.013.8333335
TGCTCGG11100.013.66666710
ATCTCCG8950.013.64245910
CGAGACT9050.013.49171323
TATACCG1254.8498463E-513.3200015
CACGAGA9450.013.11640321
CTACACT5950.013.0588244
TCTCCGA9150.012.93989111
ACTAGGC9500.012.85263227
TGCGACG1307.0056834E-512.80769322
GTAGCAC2900.012.7586213
AGGTCGC10200.012.69607835
GCGAACT10100.012.63861523
Produced by FastQC (version 0.11.5)