FastQCFastQC Report
Fri 29 Apr 2016
ERR1042687.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042687.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7466875
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT365870.48999079266761525No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT353920.47398677492257474No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT229480.3073307106386541No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG167340.22410981836444294No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT142020.190200050221813No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126000.16874529170503055No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG116800.15642420691386957No Hit
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT106110.1421076420858793No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG103940.13920147317318154No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG99170.13281325855863396No Hit
CTCTAATACTGGTGATGCTAGAGGTGATGTTTTTGGTAAACAG92580.1239876119527915No Hit
CTTCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGT91660.1227555034736754No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC88390.1183761613794258No Hit
CCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCGTT85800.11490750816104461No Hit
TCCGTACTCTGCGTTGATACCACTGCTTCCGTACTCTGCGTTG83540.11188080689712898No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGGAC19550.020.439926
CGAGACG20350.020.0909123
ATCTCGT20100.020.06467837
TATACAC22050.019.8843543
TATCTCG21500.018.84418736
ACGAGAC22100.018.75113122
AGACGGA22200.018.33333425
ATCTCCG22750.018.21538510
TCTCCGA23000.018.1782611
CCCACGA23250.017.90322519
ACGGACT22400.017.83928727
CGGACTC22850.017.6498928
TACACAT26700.017.4606745
GGGGTTA19450.017.406176
CGAGCCC24000.017.3437515
CACGAGA24350.017.09445621
TAGGGGT20600.016.8834954
CCTATCT24450.016.72188234
GAGACGG25150.016.47713724
GCCCACG26400.016.39772818
Produced by FastQC (version 0.11.5)