FastQCFastQC Report
Fri 29 Apr 2016
ERR1042686.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042686.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2847655
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT159190.559021370215142No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT139880.49121118955772386No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55920.19637210265990787No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53900.18927854673406716No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT46790.16431063453964753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC10650.020.4976523
CGCGTAA500.007037371418.510
ATCTCCG11650.017.30901110
TACACAT13250.017.1735865
TTATACA12100.016.9710752
ATCTCGT11450.016.48034937
CACGAGA12450.016.04819321
GTAGCAC2700.015.7592583
ATACACA15250.015.649184
CGAGCCC12900.015.63178315
TCTCCGA12950.015.42857311
CCCACGA12600.015.41666619
ACGAGAC13100.015.11068622
ACACATC14250.015.0596496
CGAGACT13400.015.04850723
CTTATAC13300.014.6052641
GCCCACG13650.014.366318
TAATCCG1203.3037264E-513.8749995
CATCTCG14050.013.69395136
ACATCTC15600.013.6378218
Produced by FastQC (version 0.11.5)