FastQCFastQC Report
Fri 29 Apr 2016
ERR1042684.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042684.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5156097
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT313220.6074749951368255No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT288480.5594929653185345No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT176710.34272047248141374No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103900.2015090096249159No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT10900.021.3853232
AATCTCG11450.020.19650736
ATCTCGT11050.019.58823637
CACGAGA11950.019.50627521
GCGAATC11500.019.46521633
TAAGGCG12350.018.72469729
ACGAGAC12400.018.64919322
GACTAAG12500.018.35226
CCCACGA12800.018.21093819
CGAGACT13500.017.67777823
TATACAC15750.017.5015873
AGGCGAA13750.017.4909131
ACTAAGG13250.017.31320827
TCTCCGA13500.016.99259411
CGAATCT13450.016.78066834
ATCTCCG13950.016.70967910
GCCCACG14250.016.48771918
TAGGGGT8850.016.3050844
GAGACTA14450.016.0034624
CGAGCCC14350.015.857141515
Produced by FastQC (version 0.11.5)