FastQCFastQC Report
Fri 29 Apr 2016
ERR1042683.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042683.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2465987
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47850.1940399523598462No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT38620.15661072017005767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30350.018.2866551
ATTACAC2550.015.2352943
GTATCAA40600.013.6243842
TTAACGG3450.013.40579735
TATACTG3050.013.3442615
TAATACC2102.044544E-913.2142874
ATTAGAG4950.013.0808093
TTAGCGA1451.3733712E-512.7586227
TACTCCG5100.012.6960785
GTATTAG5450.012.5596331
TCGCTAA1359.969E-512.33333414
TATTAGA5250.012.3333332
TAACGGC3800.012.17105336
CTAGACT2451.5643309E-912.0816334
GTACTAG1552.7226468E-511.9354841
TCTATAC2808.367351E-1111.8928583
TTTAACG4100.011.73170734
AGTATTA3259.094947E-1211.38461515
CTAGGAC2452.0170773E-811.326533
AACGCAG53600.011.2518655
Produced by FastQC (version 0.11.5)