FastQCFastQC Report
Fri 29 Apr 2016
ERR1042682.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042682.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9207110
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT194100.21081533727738672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT148460.16124495091293575No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG104060.11302134980466184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100480.10913305043602171No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC25950.017.1811183
TACACAT29200.015.3321915
ATCTCGT24150.014.93788937
TCTTATA44250.014.34011536
ATCTCCG28600.014.10139810
CCCACGA28000.013.74285719
CTTATAC47800.013.19769937
TCTCCGA30000.013.07333311
GTCGGGA20000.013.04252
ACGAGAC30100.012.90697822
ATACACA37350.012.8781794
TTATACA32050.012.8720742
ACATCTC33050.012.8184588
CACGAGA32200.012.18012421
TATTAGA20600.012.1237872
TTAACGG15500.011.93548535
ATACCGA4350.011.9080456
ACACATC36050.011.8543696
TTATACC8900.011.64045054
GCCCACG34200.011.63011718
Produced by FastQC (version 0.11.5)