FastQCFastQC Report
Fri 29 Apr 2016
ERR1042680.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042680.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5237409
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT88880.1697022325352097No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT72460.13835085249213877No Hit
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT71460.13644151144201264No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT64040.12227420085007681No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA105900.020.8234181
TATACAC17200.018.6075573
GTCGGGA18100.016.8646412
TACACAT19450.016.6452455
CGTACTA18750.015.78666829
GACCGTA18900.015.66137626
CTAGATC19850.015.28463533
GTACTAG19450.015.12339330
ATCTCCG19950.015.02255610
TTATACA20850.014.9064752
ATCTCGT19600.014.72448937
ACATCTC22300.014.4349788
GTATTAA5050.014.2871291
TAGATCT20850.014.28537234
ACCGTAC21150.014.25768327
GTATCAA154700.014.2188112
ACTAGAT22600.014.07964632
AGATCTC22350.013.74049235
CTTATAC22250.013.6359551
ACGAGAC22400.013.62723222
Produced by FastQC (version 0.11.5)