FastQCFastQC Report
Fri 29 Apr 2016
ERR1042679.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042679.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14024416
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT630310.4494376093806687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT627160.44719152654912686No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT382650.27284558586967184No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296510.21142413345411315No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT143190.10210050814237112No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC36600.019.0054653
ATCTCCG32650.018.24502410
GGTATCA309000.017.5300981
ATCTCGT32300.017.29721537
CCCACGA35450.017.11706519
ACGAGAC37100.016.35579522
TCTCCGA37350.015.89959811
CGAGCCC39100.015.56649615
TACACAT49250.015.1756345
GCCCACG42400.014.39858418
CTTATAC45300.014.0485651
CCGAGCC49000.013.25204114
TTATACA49700.013.1770632
ATACACA66950.012.7662434
CACGAGA49150.012.68463921
GTATCAA431600.012.537652
ACACATC53550.012.5060696
ACATCTC53350.012.4489228
GGATCTC48900.012.03067535
GATCTCG49400.012.021255536
Produced by FastQC (version 0.11.5)