FastQCFastQC Report
Fri 29 Apr 2016
ERR1042678.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042678.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6952597
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCAGAGAGGATCTCGT76890.11059176880236263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC16150.020.5046443
TACACAT18650.018.053625
TTATACA18100.017.5801122
ATACACA25500.015.3803924
GGTATCA112250.015.0801771
ATCTCGT20150.014.96526137
CTTATAC21550.014.8515081
TATTAGA23000.014.0760862
TACGTCA800.006302840513.8755
ATCTCCG23050.013.80477210
TTAGACT5000.013.694
ACGAGAC23700.013.42616122
GTATTAA4150.013.3734941
TCTAGCA20550.013.1435523
ACATCTC25500.013.1313728
TCTCCGA25000.013.09811
CCCACGA25250.012.8950519
CCTCTAG23100.012.8138531
TCGTAGC6500.012.80769314
TTAACGG19800.012.80050635
Produced by FastQC (version 0.11.5)