FastQCFastQC Report
Fri 29 Apr 2016
ERR1042677.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042677.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5678425
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT72980.1285215530714943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC14800.020.1253
TACACAT16800.018.279765
TTATACA17000.017.7382342
ATACACA21000.015.4166674
CGCGTAT1353.979876E-715.07407512
CTTATAC19400.014.9716491
TATACTG3600.014.3888885
GTCGGGA13500.014.1148162
TAGACTA2900.014.0344835
GGTATCA105650.013.5182211
ATCTCCG21950.013.4009110
ACACATC23550.013.3545656
ATACGCG1153.581036E-412.86956530
ATCTCGT22350.012.6644337
TACATCT23400.012.33333334
TATTAGA9750.011.9538462
CGACCGA7600.011.92763118
TCGCTAA2951.6370905E-1111.91525514
TCTCCGA24850.011.911468511
TTAGACG1401.4001681E-411.8928574
Produced by FastQC (version 0.11.5)