FastQCFastQC Report
Fri 29 Apr 2016
ERR1042675.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042675.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11573790
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1041470.8998521659715616No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT983420.8496957349321183No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT636400.5498630958398243No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT488830.422359486391234No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158440.136895519963642No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT120150.10381214796535967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA414300.017.2363031
ATCTCGT15300.016.68627437
ATCTCCG16750.015.24179110
GTCGGGA26300.014.3498092
CCCACGA18550.014.26145619
TTAACGG14300.014.10139835
TCTCCGA18900.013.60582111
TAACGGC14850.013.45454536
CGAGCCC19450.013.41131115
TTCGCGC1951.0290023E-813.28205230
TATACAC28400.012.83274653
ACGAGAC22000.012.69772622
GCCCACG21150.012.50827418
GTATCAA577400.012.3835312
CATCTCG21300.012.33333336
CCGAGCC25900.012.28571414
GTACGAC2002.0450352E-712.0253
GTATTAG38100.011.9934381
TAACCGG3900.011.85897422
ACCGCCT18750.011.83999912
Produced by FastQC (version 0.11.5)