FastQCFastQC Report
Fri 29 Apr 2016
ERR1042674.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042674.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5610046
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40050.025.5905111
GTATCAA61300.016.5383362
GTCGGGA10800.012.504632
TAATACT12200.012.4344264
CGAACCG1050.00264577512.33333313
TCGCTAA3001.8189894E-1212.33333314
TCTAATA10750.011.8744192
TACTCCG15900.011.6352215
TATTAGA11800.011.4449152
GGCGAAA4700.011.41489410
AATACTG13800.011.3949275
TAGACCG3900.011.38461625
CGAACTA9600.011.36979224
GCGAACT9800.011.32653123
CGGTCTA4250.011.31764620
GGTAAAC9850.011.26903535
AACGCAG99500.011.1371865
TATTAAG5000.011.0999992
ATCAACG100050.011.0759622
TTAACGG10250.011.00975635
Produced by FastQC (version 0.11.5)