FastQCFastQC Report
Fri 29 Apr 2016
ERR1042672.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042672.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10299350
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT748600.7268419851738216No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT618400.6004262404909048No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT378880.3678678751571701No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342420.3324675829057174No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118510.1150655138431066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT15650.016.78594237
TTAACGG18800.016.03989435
TAACGGC20050.015.13216936
CCCACGA19100.014.72251219
ATCTCCG18900.014.6825410
ACGAGAC20350.014.36363622
TCTCCGA19600.014.15816411
TATACAC24850.014.1448693
CGAGCCC20600.013.5606815
CGAGACT22050.013.50793623
TCGTATG4150.013.37349437
CACGAGA21650.013.33025421
GCCCACG22900.013.00655118
GACTAGG21250.012.88470626
TAATACT35550.012.8537284
GTATTAG39950.012.8272841
GGTATCA383400.012.6469741
CTCTAAT27550.012.4228681
AACGGCC25100.012.3824737
CTAATAC37950.012.3820823
Produced by FastQC (version 0.11.5)