FastQCFastQC Report
Fri 29 Apr 2016
ERR1042671.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042671.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8274255
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT861131.0407341809020874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT736430.8900257485417116No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT484950.5860950623349171No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT383450.4634254080880998No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123610.14939109321624727No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT96470.11659055709547266No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92690.11202216997179806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGACG9000.017.88333323
GACGGAC9700.016.59278326
ATCTCGT9600.015.99479137
TATCTCG10450.015.40191536
GGTATCA350000.014.9374281
ACGGACT11400.014.44298227
ATCTCCG11900.014.30252110
CCCACGA11800.014.26694919
CGAGCCC12250.014.19591915
TCTCCGA12450.013.96787211
TACCCCG8750.013.3200015
TATACTG11700.013.2820515
CGGACTC12800.013.15234528
GTCGGGA12700.013.1102362
ACGAGAC13000.012.9522
CACGAGA14050.012.50889621
TTAACGG12200.012.28278735
ATTTCGG14350.011.98954729
TAACGGC12750.011.75294236
GCCCACG14650.011.74402718
Produced by FastQC (version 0.11.5)