FastQCFastQC Report
Fri 29 Apr 2016
ERR1042670.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042670.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2438553
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT788363.2329008227420117No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT764303.134235753744126No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT546502.2410831341373347No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240580.9865686741276487No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT230700.946052843633089No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT190360.7806268717555043No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT125120.5130911651294845No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93450.383219064748644No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65360.268027801733241No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG55060.2257896383634065No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG52590.21566068073976657No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49460.2028252000264091No Hit
GAGTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTG45140.18510977616643967No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40970.168009471190497No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40410.16571302735679724No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG39030.16005393362375145No Hit
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC38480.15779849771565352No Hit
GGTATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAG38290.15701934712921967No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG33750.13840174890601106No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA33600.13778663002198435No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC32080.13155342533051365No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT31530.12929798942241566No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA31010.12716557729112304No Hit
TATCAACGCAGAGTACGGGAAGCAGTGGTATCAACGCAGAGTA30270.12413099079659126No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT28380.11638049285785464No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT27870.11428908865216382No Hit
GAGTACGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA24870.1019867109716295No Hit
GGTATCAACGCAGAGTACGGAAGCAGTGGTATCAACGCAGAGT24820.1017816713436206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC28850.030.9081441
TATACAC30500.029.9639343
TTATACA31200.028.8766042
ATACACA38100.024.4238834
TACACAT37700.024.0450945
ATCTCCG40000.022.5237510
ACACATC41050.022.2630946
ACATCTC42100.021.8396668
TCTCCGA41850.021.48387111
GCCCACG41700.021.47242218
CCCACGA41450.021.42340319
ATCTCGT38450.021.21846637
CGAGCCC42200.021.17417115
CCGAGCC42650.021.03751414
AGCCCAC43150.020.96523717
CACATCT43350.020.9538657
TCCGAGC42850.020.8961513
CTCCGAG42950.020.89057212
CATCTCC43850.020.7993149
CACGAGA41500.020.6397621
Produced by FastQC (version 0.11.5)