FastQCFastQC Report
Fri 29 Apr 2016
ERR1042669.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042669.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8457722
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT1424941.68477989699827No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT977481.155724910324553No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT682840.8073568745815953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT613350.7251952712562555No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216550.2560382098158345No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT159570.1886678233216935No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT156000.18444682859048808No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA153950.18202300808657462No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT150740.17822765988288575No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110220.13031877850797177No Hit
GTACGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA102090.12070626109489056No Hit
GAGTACGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA99060.11712373615495994No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT23250.023.55268937
ATCTCCG25900.021.78571510
CGAGACA26250.021.63618923
CCCACGA26750.021.37009419
AATCTCG26400.020.88257636
ACGAGAC28250.020.36637122
CGAGCCC27900.020.29032115
TCTCCGA28800.019.72048611
CACGAGA29150.019.67409921
GCCCACG29450.019.41086618
TATACAC31550.019.3502373
TACACAT34650.018.3131315
CCGAGCC33600.017.94940614
ACATCTC37050.016.8272618
AAATCTC34550.016.4920435
ACACATC35700.016.427176
ATACACA42600.015.8943664
TCCGAGC37650.015.47808813
GACAGGC37300.015.17694326
GAAATCT37900.015.13192734
Produced by FastQC (version 0.11.5)