FastQCFastQC Report
Fri 29 Apr 2016
ERR1042668.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042668.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7727746
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT497390.6436417553061397No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT394660.5107051913973363No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237080.30679062174144955No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT227900.29491134931194685No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77660.10049502144609825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTACTA7200.013.61805529
TTAACGG12300.012.78455335
GTATTAG25150.012.5049711
GACCGTA7550.012.49668926
TAACGGC13350.011.77902636
TAATACT23450.011.7547984
ATCTCGT8950.011.57541937
CGTCGAA1451.940948E-411.48275919
TACCCCG9550.011.429325
TATTAGA21350.011.3512882
CGTGCAT9500.011.29473710
TCTAATA22400.011.1495532
AATACTG25600.011.1289075
CTAGCGT2002.6626185E-611.14
TCGCTAA2502.7210262E-811.09999914
TATACAC13700.010.9379563
GGTATCA323750.010.921
CTAATAC28250.010.8707963
CGTTATA2053.579984E-610.82926752
GTACTAG8900.010.80898930
Produced by FastQC (version 0.11.5)