FastQCFastQC Report
Fri 29 Apr 2016
ERR1042666.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042666.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9065093
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT699100.7712000307112128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT551210.6080577441400767No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT341960.3772272385953459No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT323210.3565435015393664No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119390.13170300624604733No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGAAT12150.018.27160632
GCGAATC13800.015.81884133
CCCACGA14900.015.271811519
ATCTCGT12800.015.17578137
TAAGGCG15250.014.55737729
GACTAAG15700.014.4936326
TATACAC20950.014.2171833
AATCTCG14800.014.12536
AGGCGAA17350.013.32853131
CACGAGA17500.013.00285721
ACTAAGG18000.012.53888927
TCTCCGA18300.012.5355211
ATCTCCG18500.012.510
ACGAGAC19550.012.49104822
GAGACTA18300.012.03005524
AGACTAA18250.011.96164325
TATACTG13450.011.8289965
GCGCTTA4100.011.73170823
CGAGCCC19950.011.59147815
CGAGACT22400.011.56250123
Produced by FastQC (version 0.11.5)