FastQCFastQC Report
Fri 29 Apr 2016
ERR1042664.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042664.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4691134
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53280.11357594986627967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGTA1409.573019E-613.2142869
GTATTAG12950.013.1428581
GGTATCA73700.013.0027141
TCGGCGA2255.7207217E-912.33333313
TATTAGA11150.012.1121082
ACACCGT3400.011.9705886
TACCCCG5150.011.8543695
CGAACTA9700.011.63402124
TCTATAC3053.092282E-1111.524593
TACACTA4050.011.4197535
TTAACGG8700.011.27011435
ACCGATT5450.011.20183620
CCGATTT5650.011.13274421
CGACCGA5500.011.09999918
GTCGGGA7850.011.0764332
TAACGGC8950.010.95530836
TCGGACG5450.010.86238613
TAGACAG7700.010.8116895
TACACCG3605.456968E-1210.7916665
TAACCGG1400.001863747410.57142922
Produced by FastQC (version 0.11.5)