FastQCFastQC Report
Fri 29 Apr 2016
ERR1042663.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042663.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7325518
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT637120.8697268916682752No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT473680.6466163894485005No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT290750.39690026015907676No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273850.37383021924183385No Hit
GTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAGTG127290.17376245611573135No Hit
TTCCCGTACTCTGCGTTGATACCACTGCTTCCCGTACTCTGCG88860.1213020021246279No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83080.1134117751126951No Hit
GTACGGAAGCAGTGGTATCAACGCAGAGTACGGAAGCAGTGGT78820.10759648669213563No Hit
GAGTACGGGAAGCAGTGGTATCAACGCAGAGTACGGGAAGCAG75330.10283231847904818No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGT10900.017.65137737
ATCTCCG14550.014.62199310
CCCACGA14400.014.51736219
TATACAC17600.014.5056823
TCTCCGA15050.014.38205911
ACGAGAC15450.014.2491922
CGAGCCC15300.013.90522915
TACACAT20050.013.7481295
GCCCACG15950.013.45454518
TTAACGG14400.013.10416735
CACGAGA16100.012.98447221
TAACGGC14900.012.6644336
CTACACT15400.012.6136364
TAATACT22600.012.1969034
GTATTAG28050.012.1354721
CGAGACC17800.011.84831423
GATCTCG16850.011.74777536
TAACCGG2757.876224E-1011.43636422
TTATACA22700.011.4096922
AATACTG23200.011.4030175
Produced by FastQC (version 0.11.5)