FastQCFastQC Report
Fri 29 Apr 2016
ERR1042662.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042662.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4956264
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT568811.1476588010646729No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT486220.9810211885404007No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT258460.5214815030030685No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT231050.4661777500149306No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76510.15437030795776818No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT64510.13015852262914163No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT58980.11900092489019955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCCG12500.018.94410
GGTATCA191900.018.297551
ATCTCGT12850.018.14007837
TATACAC15500.017.9032253
CCCACGA14050.017.11743719
TCTCCGA14500.016.4586211
CGAGCCC14550.016.40206115
GTCGGGA7000.016.1214282
CATCTCG15600.015.65384736
GCCCACG15600.015.53525718
ACGAGAC15950.015.07836922
CCGAGCC17800.014.96629314
CGAGACC15850.014.94006423
CACGAGA16450.014.39513821
CTACATC16650.014.33333333
TACACAT20550.014.31386855
CTTATAC18200.014.2307691
TAACCGG1051.6573779E-414.09523822
CGGTCTA4050.013.70370420
GTATCAA256600.013.6695252
Produced by FastQC (version 0.11.5)