FastQCFastQC Report
Fri 29 Apr 2016
ERR1042661.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042661.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5097788
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC13050.017.5785433
TACACAT15150.015.1419145
TTATACA15400.015.0162332
ATACACA19400.014.5902064
TAGAACG1152.2126802E-514.4782614
TTAGACT3100.014.322584
ATCTCGT14150.013.989437
GTCGGGA13550.013.9261992
ATCTCCG15650.013.712460510
CGTTAGA1102.458956E-413.4545452
GGTATCA121450.013.3437621
CCTCTAG11900.013.2142851
TATTAGA12500.013.1719992
ATAGAAC2850.012.9824563
ACGAGAC16400.012.7469522
CCCACGA17250.012.65507219
ATTAGAG11650.012.5450643
TTTAGCG2859.094947E-1212.33333326
TATACTG4050.012.3333335
CTCTCTA17250.012.1188429
Produced by FastQC (version 0.11.5)