FastQCFastQC Report
Fri 29 Apr 2016
ERR1042660.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042660.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3420116
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT211690.6189556143709746No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT188840.5521450149644047No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94590.2765695666462775No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65360.19110462919971136No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA97050.019.9201451
GTATCAA124900.015.537632
TATACAC7600.015.0921063
AGGTCGC6350.013.98425235
GTCGCCC7000.012.68571537
GTAGCAC3100.012.5322583
CTTATAC7900.012.4113921
AACGCAG172500.012.2689865
TGCTCGG8150.012.257668510
TACACAT11500.012.2260875
CTGCTCG10050.012.1492549
GATCCGA1705.316584E-611.9705896
ACCGTGA4750.011.6842118
TAGAACT5150.011.4951464
GTATACT4200.011.4523824
ACTATCG1300.001003809211.38461527
ATCAACG187950.011.2801282
TATACTG4800.011.1770835
GTATACC4150.011.1445774
TACACTG9550.011.0418845
Produced by FastQC (version 0.11.5)