FastQCFastQC Report
Fri 29 Apr 2016
ERR1042659.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042659.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3592417
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51240.14263377553329695No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT41090.11437981726508922No Hit
GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGAT36330.10112968511172284No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT358.8730815E-426.4285712
GGTATCA69850.020.9763761
TATTCCG957.066358E-515.5789485
GTATCAA99050.014.7551742
GTCGGGA11350.014.6696032
GCGCTAG950.001245975713.6315791
TATTAGA6400.013.58593752
GTTAACG850.00940982113.0588233
GTATACG1000.001833798612.9500011
ATTAGAG6300.012.9206343
CGATTAC1153.5804638E-412.8695651
GTATTAG8100.012.7901231
TATATTG1807.475046E-712.3333335
GTGTCGA2307.925337E-912.0652186
TATACAC5100.011.9705883
TCGGACG3103.6379788E-1211.93548413
TTAACGG4650.011.93548435
CGTACGT1401.3998928E-411.89285723
AACGTTC1100.003747705811.77272726
GTAAGGT3153.6379788E-1211.74603219
Produced by FastQC (version 0.11.5)