FastQCFastQC Report
Fri 29 Apr 2016
ERR1042658.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042658.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4090366
Sequences flagged as poor quality0
Sequence length43
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG3850.014.4155845
CGTTAAC800.00630225613.8751
TCTATTC2457.2759576E-1213.5918383
TTAACGG5600.013.54464335
CTAGTAC2152.906745E-912.9069773
TAATACC2305.7661964E-1012.8695654
TATTCCG1153.580653E-412.8695655
CTAGCAT6350.012.8188974
TAACGGC6050.012.5371936
TCTATAC2102.8972863E-812.3333333
GTCGGGA4700.012.20212652
CTAGCGT1552.723675E-511.9354844
CCTCTAG9800.011.70408251
TCTAGCA7600.011.6842113
GGTATCA90450.011.6379211
ATCTCGT5750.011.58260937
GTATTAG11200.011.5625011
TATTAGA8350.011.5209582
ATACCAT5300.011.51886756
ATTAGAG8400.011.452383
Produced by FastQC (version 0.11.5)