FastQCFastQC Report
Fri 29 Apr 2016
ERR1042657.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042657.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12762474
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT379490.297348304098406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT355040.2781905765292842No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT270480.21193383038429695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162290.12716186532485788No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT151360.11859769508639156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC64150.025.4933743
CTTATAC64300.024.340591
TTATACA69250.023.3754522
TACACAT81650.019.8481315
ATACACA92150.018.7308734
ATCTCCG88850.017.38604410
ACACATC97550.016.6319856
ATCTCGT90300.016.57419837
CGAGACA96150.016.16224723
ACATCTC102400.015.8623058
TCTCCGA98650.015.84642611
AATCTCG96200.015.82692336
ACGAGAC101900.015.52257122
CACGAGA101000.015.42277121
CCCACGA101550.015.22993519
CGAGCCC103900.015.02791115
TCCGAGC106050.014.86280113
CCGAGCC107800.014.81029814
GCCCACG105550.014.79298918
AAATCTC104250.014.76450835
Produced by FastQC (version 0.11.5)