FastQCFastQC Report
Fri 29 Apr 2016
ERR1042656.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042656.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1726240
Sequences flagged as poor quality0
Sequence length43
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT301121.7443692649921219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT279031.6164032811196587No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT155170.8988900732227267No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT148530.8604249698767262No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53500.30992214292334785No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA47150.2731369913801094No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34630.2006094169987951No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32970.19099314116229493No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29640.17170266011678562No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT25530.1478936880155714No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA20140.11666975623320049No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA18210.10548938733895635No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC352.3889981E-531.7142853
GGTATCA81750.022.2452621
AAGGTCG603.727681E-521.5833345
TCTAGCG1251.8189894E-1120.7228
CGGTAGC450.00382657520.5555551
ACGGACC2900.020.4137948
CGAACGA3050.020.01639416
TATACTG2650.019.5471695
TTAGGAC1103.8549842E-818.53
CTAGCGG1501.2732926E-1118.529
ACGAACG3300.018.515
CGGGTAA2400.017.72916824
GACGGAC3350.017.6716427
CGGACCA3350.017.6716429
AAGACGG3700.017.55
GCGGGTA2450.017.36734623
AACGAAC3750.017.26666814
TACGCTG752.0680239E-417.2666665
GCGCAAG3950.016.860761
CGCAAGA3850.016.8181822
Produced by FastQC (version 0.11.5)