FastQCFastQC Report
Fri 29 Apr 2016
ERR1042654.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042654.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences293774
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT139194.737995874379625No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT112943.844451857550362No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83092.828364661270228No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44811.5253221864426396No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT33541.1416939552172758No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21500.7318550994982538No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18960.6453940784412507No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12550.4271991394745621No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12470.42447595770898716No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT10870.3700123223974892No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT7400.251894313315678No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6340.21581215492181066No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT5630.19164391675233342No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.17972999652794325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4730.16100812188961583No Hit
CTCGGGGGTCGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCG3830.13037232702689822No Hit
GAGGACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3740.12730874754062646No Hit
ACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3130.10654448657811788No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGTCCC200.001839706837.05
ACGACAT303.593016E-430.83333227
CTGTAGG250.00548976729.62
CCTCACG250.00548976729.623
AAAGCCG250.00548976729.615
AGACCGT405.9253165E-527.756
GTGTAAT405.9253165E-527.751
GGTATCA25750.027.0135921
CGACGAC358.853617E-426.42857225
TCCGACG358.853617E-426.42857223
CGACATT358.853617E-426.42857228
CGATCCT358.853617E-426.42857228
GTAGTCC358.853617E-426.4285724
GTATGGT809.713403E-1025.43753
GCGCAAG1400.025.1071431
CTTCCGA400.001927785323.12521
ATTGACG903.799869E-922.6111132
GTATCAA31100.022.4260462
TAGACAC502.695472E-422.25
GACCGTG502.695472E-422.27
Produced by FastQC (version 0.11.5)