FastQCFastQC Report
Fri 29 Apr 2016
ERR1042653.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042653.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6084138
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT699461.149645192137325No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT695811.143645985676196No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT426860.7015948684924636No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT363500.5974552188000996No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82610.13577930020653706No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82240.13517116146938155No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72850.11973758649129917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA191300.024.7956091
TCTATAC4700.017.7127673
GTATCAA278550.017.0355422
GCGATAC803.385552E-416.187528
ACGGACC8200.016.0182918
CGAACGT2250.015.6222214
GACGGAC8550.015.5789477
TATACTG9000.015.4166655
CGAACGA4850.014.87628816
AAGACGG10450.014.162685
TCTAGCG4450.014.13483228
ATTGACG9200.014.07608732
GCGTAGA1753.574496E-813.7428571
CGCAAGA10800.013.7037032
CGGACCA10700.013.6588799
CGCGCTA9150.013.34426124
CGGGTAA5850.013.28205124
GCGCAAG11400.012.9824561
ACGCGCG9700.012.96907221
TAATACT12700.012.9645674
Produced by FastQC (version 0.11.5)