FastQCFastQC Report
Fri 29 Apr 2016
ERR1042652.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042652.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4841763
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT410690.8482240869699736No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT399930.826000776989704No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT223100.46078257031581266No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT185570.3832694826244077No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA127900.020.5250191
ACGGACC6800.017.6838238
GTATCAA178950.014.6594022
GCGCAAG8650.014.5433531
GACGGAC7400.014.57
CGCGATA908.2807866E-414.38888814
CGGACCA8500.014.1470599
AAGACGG9050.013.9005535
GTGTAAG8250.013.6787881
CGAACGA2601.8189894E-1213.51923116
ATTGACG5700.013.30701732
AGACGGA8950.013.2290516
TATACTG8850.012.9604525
TTGACGG6000.012.9533
CGACGGA1153.580866E-412.86956527
CGCAAGA9350.012.8609632
CGTACAC1653.8100843E-612.3333333
AACGCAG217200.012.1374317
ACCGACC3600.011.8194458
ATTAGAC4250.011.7529413
Produced by FastQC (version 0.11.5)