FastQCFastQC Report
Fri 29 Apr 2016
ERR1042651.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1042651.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6971419
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT547460.7852920617739373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT475740.6824148713482865No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT314840.4516153741440588No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT222960.3198201112284314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136700.028.2304331
GTATCAA205700.018.7788032
TATACTG12450.015.8995985
GACGGAC8250.015.6969697
TACGACG5300.015.3584895
ACGGACC8500.015.2352948
GCGTAGA2109.094947E-1214.976191
AAGACGG10000.014.6150015
CGAACGA6000.014.18333316
ACGACGG5650.013.7522126
CGGGTAA5550.013.66666724
CGGACCA9750.013.2820519
GCGCAAG11050.013.2262451
ACGCGCG10800.013.189815521
GTGTAAG11000.013.1181821
CGCGCTA10900.013.06880824
AGTGCGC3850.012.97402610
TAATACT14350.012.8919874
TATCAAC302200.012.8618473
ATCAACG303650.012.8430764
Produced by FastQC (version 0.11.5)